Silver Hydrosol Info

   

Comparative Bacteriology Analysis

Mesosilver – Advanced Colloidal Silver (XX46A) vs. Sovereign Silver(s)

September 17, 2001

Purpose.

The purpose of this study was to compare and contrast the inhibition of the above specimen (silver preparation) with Sovereign Silver (SS) on both normal and antibiotic resistant strains of Staphylococcus aureus (“Staph”). This was accomplished by inoculating healthy Staph cultures with such silvers on standard YT plates, then placing them in a 37° C incubator overnight.

Materials and Methods.

Each 95mm sterile polystyrene plate was filled with 5 mm of YT media consisting of 0.5% sodium chloride, 0.6% yeast extract, 0.8% tryptone and 2% agar. A small 3 mm scrape of Staph was re-suspended in sterile 18 MÙ lab water and diluted. To each 100 µl dilution of staph cells, 10µl of silver was added and then left to sit for both 4 minutes and 7 minutes. After the appointed time, five 10µl spots of each diluted strain containing silver were arranged on a YT plate.

A. The YT media was autoclaved and poured into sterile plates and allowed to dry.

B. Two strains of Staph received from the New York Hospital of Queens.

1. B14192 – wildtype/ normal strain -Renamed S-1

2. B14310 – antibiotic resistant (MRSA) -Renamed S-2

C. An optical density (O.D 590) of 0.135 was achieved by using a 3 mm scrape of both S-1 and S-2, which were resuspended in 1250µl of lab pure water. Then a standard 10:1 dilution series was performed on each strain.

D. The silver products were added to each dilution of S-1 and S-2; the cultures were then agitated and allowed to sit until spotted onto the YT plate. The exposure time of each dilution to the silver was both four and seven minutes. This protocol was repeated for each of the silver formulations.

E. Three different types of silver products were used:

1. Mesosilver (XX46A)

a. ppm = 20.74
b. pH = 6.26
c. tyndall = ++++ (high tyndall effect)
d. color = pale amber

2. Sovereign Silver (SS)

a. ppm = 10.05
b. pH = 6.78
c. tyndall = + (low tyndall effect)
d. color = clear

3. Sovereign Silver (SS-20)

a. ppm = 20 .28
b. pH = 6.18
c. tyndall = +
d. color = clear

F. Positive control plate -No colloidal silver added to S-1 or S-2 cultures
Negative control plate -No Staph or silver added to check for contamination
(image not included).

G. Plates then placed in a 37°C incubator overnight.

Results.

Qualitative results can easily be seen on each plate. The positive control for S-1 grew out 4.5 spots, represented by (++++½) and the positive control for S-2 grew out 4.5 spots when colloidal silver was not present (++++½). A (-) represents no Staph grew on the plate. The effectiveness of the colloidal silver products can be compared by the number of spots or +’s seen on the graph.

Inhibition of Staph @ 4 Minutes

S-1 S-2
+ control ++++½ ++++½
XX46A ++½ ++½
Sovereign Silver + +
Sovereign Silver – 20 - -

Inhibition of Staph @ 4 Minutes

S-1 S-2
+ control
XX46A
Sovereign Silver
Sovereign Silver – 20

Inhibition of Staph @ 7 Minutes

S-1 S-2
+ control ++++½ ++++½
XX46A
Sovereign Silver - -
Sovereign Silver – 20 - -

Inhibition of Staph @ 7 Minutes

S-1 S-2
+ control
XX46A
Sovereign Silver
Sovereign Silver – 20

Discussion.

In the experiment at 4 minutes, XX46A (Mesosilver) appears to have only a marginal effect on inhibiting the growth of the Staph cultures, while Sovereign Silver at 10 ppm almost eradicated the Staph; and totally eradicated it with the 20 ppm Sovereign Silver (the same concentration as Mesosilver). In the 7 minute experiment XX46A was again outperformed by both SS and SS-20 ppm, with virtually total effectiveness against the bacteria, while XX46A left spots of Staph on the plate.